[Reed Biotech] Human Integrin beta-1(ITGB1) ELISA kit

[Reed Biotech]  Human Integrin beta-1(ITGB1) ELISA kit

 

 

Catalog Number：RE1993H

 

Product Name	Human Integrin beta-1(ITGB1) ELISA kit	Species	Human
Uniprot ID	P05556	Alternative Names	ITGB1; FNRB; MDF2; MSK12; Integrin beta-1; Fibronectin receptor subunit beta; Glycoprotein IIa; GPIIA; VLA-4 subunit beta; CD antigen CD29
Detection metho	Sandwich	Sensitivity	15.6 pg/mL
Standard	4000pg/mL	Detection Range	62.5-4000 pg/mL
Sample type	Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL
Reaction time	3.5H	Research Area	Immunology
Test principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ITGB1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ITGB1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ITGB1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ITGB1. You can calculate the concentration of Human ITGB1 in the samples by comparing the OD of the samples to the standard curve.