[Reed Biotech] Human IL-7(Interleukin 7) ELISA Kit

[Reed Biotech]  Human IL-7(Interleukin 7) ELISA Kit

Catalog Number：RE1359H

 

Product Name	Human IL-7(Interleukin 7) ELISA Kit	Species	Human
Uniprot ID	P13232	Alternative Names	IL7, IL-7, IL-7interleukin-7, interleukin 7, Lymphopoietin-1, PBGF
Detection method	Sandwich	Sensitivity	0.26 pg/mL
Standard	100pg/mL	Detection Range	1.56-100pg/mL
Sample type	Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL
Reaction time	3.5H	Research Area	Signal transduction;CD & Adhesion molecule;Tumor immunity;
Test principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-7. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-7, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-7. You can calculate the concentration of Human IL-7 in the samples by comparing the OD of the samples to the standard curve.