[Reed Biotech] Rat TGF-β2(Transforming Growth Factor Beta 2) ELISA Kit

[Reed Biotech]  Rat TGF-β2(Transforming Growth Factor Beta 2) ELISA Kit

 

Catalog Number：RE3066R

 

Product Name	Rat TGF-β2(Transforming Growth Factor Beta 2) ELISA Kit	Species	Rat
Uniprot ID	N/A	Alternative Names	TGFb2
Detection metho	Sandwich	Sensitivity	9.38 pg/mL
Standard	1000pg/mL	Detection Range	15.63-1000pg/mL
Sample type	Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL
Reaction time	3.5H	Research Area	Enzyme & Kinase;Apoptosis;
Test principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TGFb2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TGFb2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TGFb2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TGFb2. You can calculate the concentration of Rat TGFb2 in the samples by comparing the OD of the samples to the standard curve.