[Reed Biotech] Human METRN(Meteorin) ELISA Kit

[Reed Biotech]  Human METRN(Meteorin) ELISA Kit

 

Catalog Number：RE3559H

 

Product Name

Human METRN(Meteorin) ELISA Kit

 

Species

Human

 

Uniprot ID

Q9UJH8

 

Alternative Names

Meteorin

 

Detection metho

Sandwich

 

Sensitivity

46.88 pg/mL

 

Standard

5000 pg/mL

 

Detection Range

78.13-5000pg/ml

 

Sample type

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL

 

Reaction time

3.5H

 

Research AreaImmunology

Test principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human METRN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human METRN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human METRN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human METRN. You can calculate the concentration of Human METRN in the samples by comparing the OD of the samples to the standard curve.