[Reed Biotech] Mouse IDO1 (Indoleamine 2,3-dioxygenase 1) ELISA kit

[Reed Biotech]  Mouse IDO1 (Indoleamine 2,3-dioxygenase 1) ELISA kit

 

Catalog Number：RE2268M

 

Product Name	Mouse IDO1 (Indoleamine 2,3-dioxygenase 1) ELISA kit	Species	Mouse
Uniprot ID	P28776	Alternative Names	Indoleamine 2,3-dioxygenase 1,EC:1.13.11.52,IDO-1,Indoleamine-pyrrole 2,3-dioxygenase,Ido1,Ido, Indo
Detection metho	Sandwich	Sensitivity	24.9 pg/mL
Standard	4000pg/mL	Detection Range	62.5-4000 pg/mL
Sample type	Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL
Reaction time	3.5H	Research Area	CD & Adhesion molecule;Infection immunity;
Test principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IDO1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IDO1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IDO1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IDO1. You can calculate the concentration of Mouse IDO1 in the samples by comparing the OD of the samples to the standard curve.