[Genaxxon Bioscience] ready-to-use IPTG solution - 10 x 1 mL

[Genaxxon Bioscience] ready-to-use IPTG solution - 10 x 1 mL

 

Artikel-Nr.: M3187.1001

Shipping: shipped on wet ice, store at -20°C
CAS-Nr: 367-93-1

 

Technical Data:

Specifications:
Purity (of used powder): min. 99%
C9H18O5S
MW = 238.3 g/mol
inhibitor free: tested active in Lac-gene expression

application:

Blue/White screening of bacteria.

Source

synthetic

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

CAS-Nr: 367-93-1
eclass-Nr: 32-16-04-05

 

Product information "ready-to-use IPTG solution - 10 x 1 mL"

Ready-to-use 100mM IPTG solution (Isopropyl-ß-D-thiogalactopyranoside) for molecular biology research.

IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used together with X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactoside) or Bluo-Gal (5-Bromo-3-indolyl-beta-D-galactopyranoside) in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. While IPTG binds to the lacI repressor it is altering the conformation of the lacl repressor, which prevents the repression of the β-galactosidase coding gene lacZ. Thus IPTG is an inducer of the lac operon in bacteria for detection of lac+ colonies.

Shipped as 10 x 1mL solution.