[Genaxxon] GenLadder 100 bp Plus with fluroescence dye

[Genaxxon] GenLadder 100 bp Plus with fluroescence dye

 

Order number: M3237.0050

 

Product information "GenLadder 100 bp Plus with fluroescence dye"

The GenLadder 100 bp Plus with Fluroescence Dye and Loading Dye - ready-to-use is an innovation for high throughput approaches or colony screenings! Ideally suited to determine the size of double-stranded DNA between 100 and 1000 base pairs. The additional bands at 1.5kbp and 3.0kbp allow additional DNA sizes in this range to be assigned without having to use an additional 1kbp DNA marker. At the same time, the mixture also contains a fluorescent dye. Additional staining (pre- or post staining) or the addition of fluorescent dye prior to application is thus unnecessary and saves an additional pipetting step. At the same time, it is possible to check whether a PCR was successful or not before applying the PCR sample! This DNA ladder is an ideal match for our OnePCR 2X Mastermix, which also contains the fluorescent dye. Together the ideal pair for Colony Screens!

The Gene Ladder 100 bp Plus consists of 12 fragments in sizes between 100 - 1000bp spaced 100bp apart. In addition, there are further bands at 1500bp and 3000bp.

The 500bp and 1500bp fragment show stronger intensity to allow easier identification. All fragments are 'blunt-ended'. The GenLadder can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography.

Fragment sizes (in base pairs): 3000, 2x 1500, 1000, 900, 800, 700, 600, 2x 500, 400, 300, 200, 100.

The DNA marker is supplied as a 0,1mg/mL solution in10 mM Tris/HCl (pH8.0), 1,0mM EDTA including loading dye. The recommended amount of DNA marker per lane is 0,5μg (5µL).

GenLadder DNA marker are available in a ready-to-use format (premixed with 6X DNA Loading Dye: M3094 (GenLadder 100 bp Plus ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix  M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye.

The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.