[Biopredic] Differentiated HepaRG cells - HPR116

[Biopredic] Differentiated HepaRG cells - HPR116

 

 

 

Key advantages of HPR116

• Time-Saving - HepaRG™ differentiated cells are ready-to-use
• Robust - hepatocyte differentiation level reproducible between batches
• Reproducible - cells from every tube have the same behavior
• Easy to use - well adapted to different formats, matrices and supports
• Long living - HepaRG™ cell cultures remain viable and usable for at least 2 weeks
• Suitable for both acute and chronic studies
• Responses and fuctions similar to primary human hepatocytes across a range of applications

Protocol - HPR116

	1 mL / 8 million cells / 10% DMSO
Thawing process (e.g: 1 vial for plating into 24 MW-plate)
1.	Thaw and dilute cells with 9 mL of thawing medium (10 ml / 8 million cells)
2.	Centrifuge, wash and re-suspend cells with 5 mL thawing / plating medium
3.	Count cell viability, concentration and yield
4.	Adjust cell concentration to 0.96 million cells/mL  and plate cells onto 24 MW-plate  (0.5 mL / well)

Download Product Specification Sheet

Download User Guide

Quality controls include

• Number of viable cells per vial
• Post-thaw viability
• Cell confluence 6 hours after thawing and seeding cells ≥85%
• Cell morphology
• Mycoplasma detection (negative)
• Microbial sterility
• Enzymatic activities
• Inducibility of CYP1A2, CYP3A4/5, CYP2B6 at day 3 (48 hours induction)
• Uptake transporter activities

Storage and delivery conditions for HPR116 cells

Differentiated HepaRG™ cryopreserved cells are delivered in dry ice or in a nitrogen vapour dewar. If you receive them in dry ice, ensure shipment time did not exceed 48 hours.
HPR116 cells can be stored for 5 years in liquid nitrogen.

Cell morphology

Morphology of the cells after thawing in 96-well plates.
From day 1 to day 2 in 670 Thawing/ Plating/ General Purpose HepaRG™ medium.
From day 2 to day 8: in 620 Maintenance/ Metabolism HepaRG™ medium.

Day 0 / 4h after plating	Day 1
Day 3	Day 4
Day 6	Day 7

HPR116 – Cells, plates, media and supplements

• HPR116-TA08 - Cryopreserved HepaRG™ cells
  1 mL in vial with ≥8 x 106 viable cells. One vial will fully seed one 96-well plate (72,000 cells/well) and 3 vials will fully seed one 24-well plate (480,000 cells/well)

The following supplements are available from Biopredic International:

• MIL600C - Basal hepatic cell medium 100 ml
• ADD620C - HepaRG™ Maintenance/Metabolism Medium Supplement with antibiotics
  15.3 mL. When combined with 100 ml of base medium, sufficient for 12 medium changes for studies where prolonged cell life or enhanced metabolic function is required
• ADD640C - HepaRG™ Induction Medium Supplement with antibiotics
  3.7 mL. When combined with 100 ml of base medium, sufficient for 10 medium changes for induction studies
• ADD650C - HepaRG™ Serum-free Induction Medium Supplement with antibiotics
  1.6 mL. When combined with 100 ml of base medium, sufficient for 10 medium changes for induction studies
• ADD670C - HepaRG™ Thawing/Plating/General Purpose Medium Supplement with antibiotics
  13.8 mL. When combined with 100 ml of base medium, sufficient to thaw 4 vials

The following plates are provided by Biopredic International with HPR116 cells, with a proprietary coating process to ensure proper seeding and culture of the HPR116:

• PLA135C - 6-well plate coated with collagen I
  1 plate
• PLA138C - 12-well plate coated with collagen I
  1 plate 
• PLA137C - 24-well plate coated with collagen I
  1 plate  
• PLA139C - 48-well plate coated with collagen I
  1 plate 
• PLA136C - 96-well plate coated with collagen I
  1 plate 

Media per application with HPR116

Media	Catalogue reference	Renewal of the medium	Use
HepaRG™ Thawing/Plating/General Purpose Medium	MIL600C + ADD670C	Every 2-3 days	- Thawing - Seeding - For applications requiring maintaining of basal hepatocyte-specific functions
HepaRG™ Maintenance/Metabolism Medium	MIL600C + ADD620C	Every 2-3 days	- Maintenance and use of the cells until the limit date of use (3 weeks after seeding). - Maintains hepatocyte specific functions to higher levels.
HepaRG™ Induction Medium	MIL600C + ADD640C	Daily following use of  670	- Use for induction studies, up to 3 days
HepaRG™ Serum-free Induction Medium	MIL600C + ADD650C	Daily following use of  670	- Use for induction studies, up to 3 days