[Reed Biotech] Mouse IL-12 p40(Interleukin 12 p40) ELISA Kit

[Reed Biotech] Mouse IL-12 p40(Interleukin 12 p40) ELISA Kit

 

Catalog Number：RE1076M

 

Product Name

Mouse IL-12 p40(Interleukin 12 p40) ELISA Kit

Species

Mouse

Uniprot ID

P43432

Alternative Names

CLMF p40; CLMF; CLMF2; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL12 p40; IL-12 p40; IL-12 subunit p40; IL12B; IL-12B; IL-12BNK cell stimulatory factor chain 2; interleukin 12, p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40); interleukin-12 beta chain; interleukin-12 subunit beta; natural killer cell stimulatory factor, 40 kD subunit; NKSF; NKSF2; NKSF2IL12, subunit p40

Detection method

Sandwich

Sensitivity

0.47 pg/mL

Standard

250pg/mL

Detection Range

3.91-250pg/mL

Sample type

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL

Reaction time

3.5H

Research Area

Signal transduction;Metabolic pathway;Endocrinology;

Test principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-12 p40. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-12 p40 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-12 p40, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-12 p40. You can calculate the concentration of Mouse IL-12 p40 in the samples by comparing the OD of the samples to the standard curve.