[Reed Biotech] Human High Sensitivity IL-17A(Interleukin 17) ELISA Kit

[Reed Biotech]  Human High Sensitivity IL-17A(Interleukin 17) ELISA Kit

 

Catalog Number：RE2867HG

 

Product Name	Human High Sensitivity IL-17A(Interleukin 17) ELISA Kit	Species	Human
Uniprot ID	Q16552	Alternative Names	CTLA8, CTLA-8, CTLA8cytotoxic T-lymphocyte-associated serine esterase 8, Cytotoxic T-lymphocyte-associated antigen 8, IL17, IL-17, IL17A, IL-17A, IL-17A, cytotoxic T-lymphocyte-associated protein 8, CTLA-8, interleukin-17A, interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
Detection method	Sandwich	Sensitivity	0.09 pg/mL
Standard	100pg/mL	Detection Range	1.56-100pg/mL
Sample type	Serum, Plasma and Other biological fluids,Sample Volume= 100μL
Reaction time	3.5H	Research Area	Cancer, Immunology;
Test principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-17A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-17A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-17A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-17A. You can calculate the concentration of Human IL-17A in the samples by comparing the OD of the samples to the standard curve.

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

 

100.00	1.678	1.650
50.00	1.091	1.063
25.00	0.702	0.674
12.50	0.348	0.320
6.25	0.207	0.179
3.13	0.090	0.062
1.56	0.054	0.026
0.00	0.028	0.000

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

 

Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(pg/mL)	48.63	57.44	12.85	37.85	33.41	60.26
Standard deviation	2.53	23.38	28.80	2.34	21.52	50.36
C V (%)	6.53	4.24	4.95	6.87	7.88	7.15

Rate of recovery

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample TypeRange(%)Average Recovery(%)

Serum (n=8)	80-93	85
EDTA plasma (n=8)	87-99	93
Cell culture media (n=8)	90-103	96

Linear

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Serum (n=5)EDTA plasma (n=5)Cell culture media (n=5)

1:2	Range (%)	87-97	80-95	89-103
	Average (%)	90	90	92
1:4	Range (%)	86-92	80-95	85-102
	Average (%)	90	89	93
1:8	Range (%)	89-103	87-96	96-104
	Average (%)	92	92	93
1:16	Range (%)	89-98	82-95	87-98
	Average (%)	91	90	91