[Profacgen] Recombinant Rat Cd47 Protein(His tag) (RH0267CL)
Cat.No.
RH0267CL
Synonyms
CD47; integrin-associated protein
Species
Rat
Accession
Tag
His
Predicted N Terminal
Gln 19
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.
Bio-activity
Measured by its binding ability in a functional ELISA. Immobilized Rat CD47-His at 10 μg/ml (100 μl/well) can bind mouse SIRPA-Fc, The EC50 of mouse SIRPA-Fc is 0.6-1.4 μg/ml.
Molecular Mass
Recombinant Rat CD47 comprises 133 amino acids and predicts a molecular mass of 15.2 kDa. The apparent molecular mass of Recombinant protein is approximately 27-35 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 90 % by SDS-PAGE.
BACKGROUND
Background
Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection (By similarity). Plays an important role in memory formation and synaptic plasticity in the hippocampus.
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