[iCell Bioscience] Human liver cancer cells, PLC/PRF/5

[iCell Bioscience] Human liver cancer cells, PLC/PRF/5

 

Catalog No.

iCell-h264

 

Country of Manufacture

China

 

Product Code

PLC/PRF/5

 

Size/Quantity

Product Use

 

1)For research use only.

2)It is not approved for human or animal use.

3)It is not approved for application in in vitro diagnostic procedures.

 

Storage

1).1ml of cryopreserved cell suspension is loaded in a 1.8ml cryovial and transported in a dry ice filled foam incubator. Cells should be thawed as soon as possible upon receiving. If it is impossible to initiating the culture immediately, the cryopreserved cells can be stored at -80°C for 1 month.

2).T-25 culture flask will be shipped at room temperature after being filled with complete culture medium. Please observe the growth of the cells under microscope upon receiving. Subculture at once when the culture reaches above 85% confluence. For the adherent cells, if the suspension cells keep growing, place the flask into the incubator for overnight stationary culture, which can help the living suspension cells to grow adhering to the wall once again.

 

Shipping Info

Dry ice(cryopreserved vial)/Room temperature(T-25 flask)

 

 

Product description

This cell line secretes Hepatitis B surface antigen (HBsAg). This cell line was originally contaminated with Mycoplasma, and then BM-cycline was used to remove Mycoplasma. The cell line has passed mycoplasma testing in our laboratory.

Product characteristics

1. Tissue: Human, liver cancer, Alexander cell

2. Cell growth pattern: Epithelioid cells, polygon; adherent culture.

3. Size/Quantity: Each vial contains >1 × 10^6 cells in 1ml volume

4. Contamination: Mycoplasma, bacteria, yeast, and fungi tests are negative.

5. Packaging: 1ml of cryopreserved cell suspension or T-25 flask.

Cell culture instructions Preparation

1. Preparing MEM (recommended iCell-0012) medium; high quality fetal bovine serum, 10%; 100× Penicillin-Streptomycin Solution, 1%.

2. Culture conditions: gas phase: air, 95%; CO2, 5%. Temperature: 37℃, incubator humidity: 70% - 80%.

3. Cryopreservation solution: 90% FBS, 10% DMSO, prepare it when it needs to be used.