[Reed Biotech] Mouse MLKL (Mixed Lineage Kinase Domain Like Protein) ELISA Kit
Catalog Number：RE5177M
| Product Name | Mouse MLKL (Mixed Lineage Kinase Domain Like Protein) ELISA Kit | Species | Mouse |
| Uniprot ID | Q9D2Y4 | Alternative Names | Mixed lineage kinase domain-like protein,hMLKL,MLKL |
| Detection method | Sandwich | Sensitivity | 0.19 ng/mL |
| Standard | 20ng/mL | Detection Range | 0.31-20ng/mL |
| Sample type | Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids | ||
| Reaction time | 3.5H | Research Area | Metabolic pathway; |
| Test principle | This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MLKL .Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MLKL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MLKL ,biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MLKL You can calculate the concentration of Mouse MLKL in the samples by comparing the OD of the samples to the standard curve. | ||
