[Biomark Labs] Ampicillin
Product Code No.:
BD003
COLOUR: White discs of 6 mm diameter with abbreviated name “A”.
DIRECTIONS:
1. Prepare sterile plates of Mueller Hinton Agar (B263).
2. Prepare inoculum as per Bauer-Kirby Method-make a pure inoculum of culture. Inoculate pure single colony of test organism – E. coli (ATCC 25922), S. aureus (ATCC 25923) and P. aeruginosa (ATCC 27853) in 5ml Pre tested sterile SCDM (B040) in a test tube & incubate at 36-37oC for 2 hours. Compare the inoculum turbidity with that of standard 0.5McFARLAND STANDARD (BA 132).
3. Dip a sterile swab into the standardized inoculum and press against the wall of the test tube to remove excess liquid. 4. Swab the plates by rotating in all directions till the plate becomes dry.
5. Place the antimicrobial ring on the swabbed plates aseptically with the help of alcohol dipped flame sterilized forceps. Press the ring properly on the plates.
6. Incubate the plates at 36-37oC for 18- 24 hours.
7. Measure the zones showing complete inhibition and record the diameters of the zones to the nearest millimetre using a calibrated instrument like zone scales.
USE: It is used for antimicrobial sensitivity for relevant test organisms for laboratory diagnosis. -- -- --
Precautions :For Laboratory Use.
Storage: -20oC to 8°C
Packing:50 discs in vial