[PlantcellTechnology] Plant Preservative Mixture (PPM™)

[PlantcellTechnology] Plant Preservative Mixture (PPM™)

Product Name	Plant Preservative Mixture (PPM™)
Synonyms	PPM™
Feature	Autoclavable and heat stable
Use	Preservative for plant tissue culture media
pH	5 - 7
Capacity	Available in different volumes of 30mL, 100mL, 250mL, 500mL, and 1L.
Shape	Cylindrical container
Material	Liquid
Color	Clear to light amber
Odor	Mild
Solubility	Infinite in water
Suitability	For Tissue Culture

Storage Temp	39° F (4° C).
Shelf Life	Check the product label for expiration date
HS Code	3808.94

Applications

• Controller of microbial airborne contamination.
• Manager of microbial waterborne contamination.
• Inhibitor of endogenous contamination.
• Enhancer of the success of culture experiments.
• Maintainer of the viability of in vitro seed germination.
• Promoter of callus proliferation.
• Supporter of callus regeneration.
• Ensurer of the stability of tissue culture media.

Plant Preservative Mixture (PPM™), is a broad-spectrum biocide for plant tissue culture. PPM™ is the ultimate solution to the never-ending struggle against microbial airborne, waterborne, and endogenous contamination.

Coupled with proper observation of aseptic laboratory techniques, PPM™ can increase your chances of a successful and thriving culture experiment.

The Benefits of Plant Preservative Mixture

• At optimum doses, PPM™, which stands for Plant Preservative Mixture™, is an extremely effective Preservative/Biocide, yet does not impair in vitro seed germination, callus proliferation and callus regeneration.
• PPM™ prevents the germination of both bacteria and fungi spores. It is heat stable and therefore can be autoclaved with the media.
• PPM™ can be, and should be used as a standard ingredient in plant tissue culture media, and is also substantially less expensive than commonly used antibiotics.
• While PPM™ was principally designed to inhibit airborne contamination, waterborne contamination, and contamination introduced from human contact, it can also -- in many cases -- be used to reduce endogenous contamination.
• PPM™ does not affect the genetic profile of the plants.

How To Use Plant Preservative Mixture?

1. General Dosage levels:

With the exception of endogenous contamination, the recommended dose range is 0.05%-0.2%. (For callus proliferation, organogenesis and embryogenesis, the recommended range is 0.05-0.075%.) To eliminate higher endogenous contamination densities, higher doses of PPM™ are needed.

1. Endogenous Contamination:

(a) For explants: gently and routinely shake / stir 1 cm. long explants (or shorter) for 4-12 hours in 4-5% v/v PPM™ solution supplemented as above with 3x MS
strength basal salts without Tween 20.Without rinsing, insert into a medium supplemented with 0.05 - 0.1% PPM™ for herbaceous plants and 0.2% PPM™ for woody plants.  

Note: Paragraphs 6(b) through 10 below are intended for ornamental plants only.

(b)For tubers, bulbs and scales: shake / stir the entire tuber/ bulb / scale in bleach. Rinse with water (can be done under non-sterile conditions). Slice the tuber / bulb /scale to thin slices. Shake / stir for 12-24 hours in 4- 5% PPM™ solution supplemented with full strength basal salts without pH ing & Tween 20. Without rinsing, insert into a medium supplemented with 0.1 -0.2% PPM™.