[TM Media] TRM 360 – BLOOD AGAR BASE (INFUSION AGAR)

[TM Media] TRM 360 – BLOOD AGAR BASE (INFUSION AGAR)

TRM360

​

Blood agar base (infusion agar) is used for isolation, cultivation of fastidious pathogenic microorganisms after addition of blood. This media can be used as general purpose media also, without blood addition. Blood agar base medium can also be used for primary isolation of Haemophilus species, where defibrinated sheep blood is used to enrich the medium. On addition of blood the pH value of 6.8 stabilizes and favours the formation of clear hemolysis zones. Fresh, defibrinated sheep blood is most suitable for determent hemolysis forms. It gives best results for Group A Streptococci. But sheep blood fails to support growth of Haemophilus haemolyticus due to deficiency of pyridine nucleotides. However, addition of horse blood supports H. haemolyticus growth and haemolysis and the growing colonies mimic Streptococcus pyogenes.

​

Medium contains a rich nutrient base, which provides optimal growth conditions for all relevant microorganisms. Tryptone and Brain Heart infusion supplies amino acids, minerals and other essential growth factors in the medium for the growth of microorganisms. Sodium chloride maintains the osmotic balance of the medium. Agar is a solidifying agent.

​

INSTRUCTION FOR USE

 Gently heat the vial with gentle swirling to melt the medium completely.

 Cool to 45 - 50°C and aseptically add 5-7% (v/v) sterile defibrinated blood.

 Mix properly and dispense sterile into Petri plates.

​

QUALITY CONTROL SPECIFICATIONS

Appearance of Powder Basal medium: Light amber colour, clear to slightly opalescent gel After addition of blood: Cherry red colour, opaque gel pH (at 25°C) : 7.3 ± 0.2

​

​

#TMMedia #TRM360 #BLOODAGARBASE #AgarBase