[Reed Biotech] MF-Mouse PAI-1(Plasminogen Activator Inhibitor 1) ELISA Kit

[Reed Biotech]  MF-Mouse PAI-1(Plasminogen Activator Inhibitor 1) ELISA Kit

Catalog Number：RE2235MF

Product Name	MF-Mouse PAI-1(Plasminogen Activator Inhibitor 1) ELISA Kit	Species	Mouse
Uniprot ID	P22777	Alternative Names	SERPINE1, PAI, PAI1, PLANH1, Endothelial Plasminogen Activator Inhibitor
Detection metho	Sandwich	Sensitivity	0.47 ng/mL
Standard	50ng/mL	Detection Range	0.79-50ng/mL
Sample type	Serum, Plasma and Tissue homogenate and other biological samples；Sample volume：25μL
Reaction time	3H	Research Area	Cell biology, Cancer, Metabolism ;
Test principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PAI-1. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Mouse PAI-1. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PAI-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Mouse PAI-1. You can calculate the concentration of Mouse PAI-1 in the samples by comparing the OD of the samples to the standard curve.

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

 

50.00	2.471	2.435
25.00	1.615	1.579
12.50	0.949	0.913
6.25	0.430	0.394
3.13	0.221	0.185
1.57	0.123	0.087
0.79	0.071	0.035
0.00	0.036	0.000

 

 

 

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Intra-assay PrecisionInter-assay Precision

Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(ng/mL)	1.51	12.42	14.81	0.99	11.76	14.37
Standard deviation	0.05	0.56	0.91	0.04	0.51	1.07
C V (%)	5.87	5.95	7.10	3.43	5.45	3.29

 

 

Rate of recovery

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Serum (n=8)	82-95	88
EDTA plasma (n=8)	94-105	98
Cell culture media (n=8)	94-107	100