T-2 toxin is a mycotoxin produced by a variety of Fusarium species. It mainly pollutes wheat, barley, corn and other food crops and their products, and poses a great harm to human health and animal husbandry. T-2 toxin immunoaffinity column can selectively adsorb the T-2 toxin in the sample solution to specifically purify T-2 toxin. The purified sample solution can be directly used in the liquid phase test.