[Genaxxon Bioscience] GreenMasterMix FAST with 50nM ROX for qPCR

[Genaxxon Bioscience]  GreenMasterMix FAST with 50nM ROX for qPCR

 

Order number: M3154.0200

 

Product information "GreenMasterMix FAST with 50nM ROX for qPCR"

GreenMasterMix FAST with 50nM ROX is optimised for realtime PCR assays in block systems. This 2X Mastermix is ready-to-use and contains all components for a successful and reliable qPCR with the exception of primer and template DNA.

Advantages of the HotStart Taq DNA polymerase used in the Genaxxon GreenMasterMix:

• Hotstart technology enables setup of PCR mixture at room temperature.
• short initial denaturing time of not more than 2 minutes
• No pipetting on ice necessary anymore
• No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days.
• Amplification of GC-rich templates
• High yields
• No Primer dimers

The Genaxxon SuperHot Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity:

1. The chemical modification inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 
2. "High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results.

The Green qPCR master mix with 50nM ROX contains all the necessary components in an optimized composition to carry out quantitative PCR:

• chemically modified Taq DNA Polymerase
• dATP, dCTP, dGTP, dTTP
• intercalating green fluorescent dye
• optimized reaction buffer
• stabilizers and enhancers to enable even amplification of low copy number targets
• 50nM internal passive reference fluorescence dye
• the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote)

This Mastermix is specially suited for the following instruments: Applied Biosystems® 7500, 7500 Fast and ViiA™ 7, QuantStudio™ instruments, Agilent Mx3000P™, Mx3005P™, Mx4000™ and AriaMx.

Other realtime PCR master mixes from Genaxxon are: M3023 - GreenMastermix No ROX >, M3045 - ProbeMastermix No ROX >, M3011 - GreenMastermix Low ROX >, M3031 - ProbeMastermix Low ROX >, M3052 - GreenMastermix High ROX >, M3010 - ProbeMastermix High ROX >.

Test sample available! The test sample price will be refunded on the first official order of the product.