[Trenzyme] greenTEV Cleavage Kit

[Trenzyme] greenTEV Cleavage Kit

SKU: P2020-CK1 

 

Including

• 1 x greenTEV (P2020-142)
• 1 x Cleavage and tag control protein (P2020-141)

Performance of cleavage conditions can be easily controlled and visualized by SDS-PAGE using the Cleavage and tag control protein. TEV cleavage results in two cleavage products of 42.8 kDa and 47.7 kDa.
The optimized greenTEV protease and the Cleavage and tag control protein are also available separately.

greenTEV is an optimized TEV protease (Tobacco Etch Virus Protease) fused to a GFP protein. greenTEV contains a His-Tag to facilitate the removal of the protease from the cleavage reaction after completion of cleavage. The removal of greenTEV can be monitored easily by following the fluorescence - this easy, fast and sensitive method omits time-consuming SDS-PAGE or Western blot analysis. The recognition sequence with the highest catalytic activity is ENLYFQ(G/S).

Overview

• Product Name: greenTEV & Cleavage and tag control protein
• Catalog No.: P2020-CK1 / CK2
• RefSeq Links: UniProt: Q0GDU8
• Synonyms: TEV Protease; TEV; Tobacco Etch Virus nuclear-inclusion-a endopeptidase; rTEV; P1 protease; EC 3.4.22.44; control protein, reference protein, cleavage control protein, multiple tag control protein, universal reference protein