[Bio World] Arachis hypogaea Lectin (PNA) - HRP (Horseradish Peroxidase)

[Bio World] Arachis hypogaea Lectin (PNA) - HRP (Horseradish Peroxidase)

 

Shelf life

2 years

Storage Temperature

2-8°C

ECCN #

EAR99

Hazmat Ship

Non-Hazardous

Purity

High Purity Grade

Appearance color

Light Yellow to Orange

Appearance form

Liquid

Abbreviation (Lectins Only):

PNA

Source

Peanut

Blood Group Specificity

T antigen (M, N)

Carbohydrate Specificity

Galβ3GalNAc

Storage Buffer

10 mM HEPES, 150 mM NaCl, 0.1 mM Ions w/ 0.02% Thimerosal

Conjugate/Tag/Matrix

Horseradish Peroxidase (HRP)

Inhibitory Carbohydrate

Galactose

Divalent Ions

Ca++, Mg++

Cancer Biomarker

Colon Cancer, Genitourinary Tract Tumor, Endometrial Carcinoma, Pancreatic & Gastric Cancer

Mitogenic Activity

No

 

Arachis hypogaea agglutinin (PNA) is purified by affinity chromatography (SKU: 21510000) and is consisting of four subunits of approximately 27,000 each. This lectin has an isoelectric point between pH 5.5 and pH 6.5. Galactose and lactose are poor inhibitors while disaccharide Gal ß1-3GalNAc and desialylated human erythrocyte N-substance (T-antigen which is present in many M and N blood groups) are potent inhibitors of PNA. Although extracts of Arachis hypogaea do not agglutinate untreated or trypsin-treated human erythrocytes, PNA has been known to agglutinate neuraminidase-treated human red blood cells. PNA may be used for clinical determination of T-polyagglutinability of erythrocytes and probing cell membranes of normal and malignant cells. This lectin can be of use in characterization of tumor-specific antigens on the surface of malignant cells. It can be employed in the fractionation of stem cells in mice for use in bone marrow transplantation across histocompatibility barriers.

Horseradish peroxidase (HRP) is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or release of light as a byproduct of the reaction. It is most commonly used for blotting, immunoassays and immunohistochemistry methods. Arachis hypogaea Lectin is conjugated to HRP at an appropriate ratio to provide optimal staining characteristics.

Specifications:
• Source: Arachis hypogaea (Peanut)
• Activity: Less than 1 μg/ml will agglutinate human erythrocytes after neuraminidase treatment of the cells.
• Carbohydrate Specificity: Terminal ß-Galactose
• Inhibitory Carbohydrate: Galactose
• Divalent Ions Required: Ca2+, Mg2+
• Conjugation: HRP

Storage and Stability:
Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8 °C for short term use. Clarify by centrifugation, if needed.

Application

Immunohistochemistry, Blotting, Glycobiology

References

Chacko, B. K., & Appukuttan, P. S. (2001). Peanut (Arachis hypogaea) lectin recognizes alpha-linked galactose, but not N-acetyl lactosamine in N-linked oligosaccharide terminals. International journal of biological macromolecules, 28(5), 365–371. https://doi.org/10.1016/s0141-8130(01)00139-8

Hennigar, L. M., Hennigar, R. A., & Schulte, B. A. (1987). Histochemical specificity of beta-galactose binding lectins from Arachis hypogaea (peanut) and Ricinus communis (castor bean). Stain technology, 62(5), 317–325. https://doi.org/10.3109/10520298709108016