[Chondrex] Arthrogen-CIA® 5-Clone Cocktail Kit, 10 mg
| Catalog Number | 53010 |
| Quantity | 10 mg (*5+ mice) |
DESCRIPTION:
Mouse anti-type II collagen 5-clone monoclonal antibody cocktail kit with LPS from E. Coli O111:B4
APPLICATION:
Use for collagen antibody induced arthritis (CAIA) in mice
QUANTITY:
Cocktail: 10 mg/ml x 1 ml
LPS: 0.5 mg/ml x 1.5 ml
FORM:
A cocktail of 5 monoclonal antibodies dissolved in 0.05M phosphate buffered saline, pH 7.4 which recognize the conserved epitopes on various species of type II collagen: clone A2-10 (IgG2a), F10-21 (IgG2a), D8-6 (IgG2a), D1-2G (IgG2b), and D2-112 (IgG2b). Clones A2-10, D1-2G, and D2-112 recognize individual epitopes clustered within the 167 amino acid peptide fragment called LyC1 (124-290) of the CB11 fragment (124-402).
Clones F10-21 and D8-6 recognize epitopes within the 83 amino acid peptide fragment called LyC2 (291-374) of the CB11 fragment.
SOURCE:
Mouse
CROSS-REACTIVITY:
Cross-reacts to most species of type II collagen including mouse, porcine, chick*, bovine, human, rat, monkey, rabbit, equine, and dog. *D2-112 does not cross-react with chick type II collagen.
ANIMALS:
7-8 weeks or older, high responder mice: DBA/1, Balb/c, B10.RIII, C.B-17, scid/scid, or 129/Sv. Low responder mice: C57BL/6 or C57BL/6 background. Sensitivity to CAIA may vary depending on mouse vendor and facility. Specific pathogen free (SPF) housing conditions are strongly recommended over conventional housing conditions as bacterial contamination may reduce the immune response of the animals resulting in an attenuated arthritis. Therefore, running a pilot study using a small number of animals before conducting a large-scale study is strongly recommended. Please contact Chondrex, Inc. customer support (support@chondrex.com) for guidance regarding animals and housing conditions.
USAGE: Administration of the monoclonal antibody cocktail by IV injection (i.e. tail vein) is recommended; however, intraperitoneal (IP) injection may also be used. Moreover, if LPS use is not desired, please note that the severity of arthritis tends to be lower.
