[Creative Biomart] ES-D3 (mouse pluripotent embryonic stem cell) whole cell lysate
| Cat.No. : | ES-D3-573M |
| Species : | Mouse |
| Tissue Type : | Embryonic |
| Preparation method : | Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). |
| Size : | 50 ug |
| Recommended Usage : | Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
| Storage Buffer : | In modified RIPA Lysis Buffer. |
| Applications : | Western Blot;Immunoprecipitation; |
